Monosodium Glutamate Plus Artemether-lumefantrine Overdose Altered Malondialdehyde, Total Protein and Albumin Concentration in Rats

Authors

  • Anthony Cemaluk Chinedum Egbuonu Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria https://orcid.org/0000-0001-5974-415X
  • Emmanuel Obi Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Chinedu P Nwuke Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Cynthia Uchechi Simon Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Justina Utodinachi Oleghibe Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Nnaemeka Raymond Ezenwafor Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Ebere Mercy Chukwu Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture Umudike

DOI:

https://doi.org/10.21467/ajgr.7.1.70-79

Abstract

This study aimed at assessing alterations in malondialdehyde, MDA, total protein and albumin concentration in the serum and liver homogenate of monosodium glutamate (MSG)-challenged rats co-treated with artemether-lumefantrine, AL. Methods involving colourimetric estimation were employed in thirty rats randomly grouped into six (n = 5) and for seven consecutive days, fed feed and water (Group A), AL therapeutic dose (Group B), AL overdose (therapeutic dose × 5) (Group C), MSG (8000 mg/kg body weight) (Group D), AL therapeutic dose plus MSG (Group E) or AL overdose plus MSG (Group F). Total protein concentration (2.64±0.09 g/dL, 2.81±0.14g/dL, respectively) in the liver homogenate of rats exposed to MSG (group D) or MSG plus AL overdose (group F) and malondialdehyde concentration in the liver homogenate of MSG plus AL overdose-fed rats (0.45±0.04 mg/ml) lowered (P<0.05) as against other groups. However, serum albumin concentration in MSG (2.59±0.13 g/dl) or AL overdose plus MSG (3.24±0.12 g/dl) fed rats was higher (P<0.05) compared to the control (2.02±0.04 g/dl). The Total protein: Albumin ratio lowered while the Albumin: total protein ratio increased in rats in MSG, AL overdose plus MSG or AL overdose groups compared with the control. Thus, the apparent MSG plus AL overdose-induced adverse influence on the studied parameters and samples of non-malarial infested rats could be via compromised liver-mediated protein metabolism capacity and bio-functions following possibly enhanced protein-malondialdehyde adduct formation in the rats.

Keywords:

Artemesinin-based, therapeutic dose, liver homogenate, malondialdehyde, albumin, total protein

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References

A. A. Amal, S. M. Marwa, and A. A. Mona, Effect of honey on monosodium glutamate induced nephrotoxicity. J Am Sci, vol. 8, Issue 1, pp. 146–56, 2012.

H. O. Akanya, S. Peter, and I. F. Ossamulu, Evaluation of the changes in some liver function and haematological parameters in monosodium glutamate fed rats. Intl J Biochem Res and Rev, vol. 6, Issue 3, pp. 113–20, 2015.

A. J. Ajibade, P. B. Fakunle, and A. A. Mene, et al., Some cardioprotective effects of aqueous extract of ginger against monosodium glutamate induced toxicity in the heart of male Wistar rats. Intl J of Recent Sci Res, vol. 4, Issue 6, pp. 972–78, 2013.

M. E. Afaf, and Z. A. E. Somaia, Protective role of ginkgo biloba on monosodium glutamate: Induced liver and kidney toxicity in rats. Res J of Pharmaceut, Biol and Chem Sci, vol. 6, Issue 1, pp. 1433-41, 2015.

K. Niaz, E. Zaplatic, and J. Spoor, (2018). Extensive use of monosodium glutamate: A threat to public health? Exptl and Clin Sci J, EXCLI J, vol. 17. pp. 273–78. 2018.

A. A. Madiha, A. A. Abeer, and F. A. Ellah, et al., The possible ameliorative effect of propolis in rats liver treated with monosodium glutamate. Natural Science, vol. 10, Issue 2012, pp. 209-19, 2012.

T. S. Mariyamma, K. S. Sujatha, and S. George, Protective effect of Piper longumLinn on monosodium glutamate-induced oxidative stress in rats.Indian J ExpBiol, vol. 47, pp. 186-92, 2009.

O. O. Okwudiri, C. S. Alisi, and A. P. Ihetuge, Monosodium glutamate induces oxidative stress and affects glucose metabolism in the kidney of rats. Intl J Biochem Res & Rev, vol. 2, pp. 1–11, 2012.

A. C. C. Egbuonu, C. A. Ezeokonkwo, and P. M. Ejikeme, et al. Some biochemical effects of sub-acute oral administration of L-arginine on monosodium glutamate fed Wistar albino rats 2: Serum alkaline phosphatase, total acid phosphatase and aspartate aminotransferase activities.Asian J Biochem, vol. 5, Issue 2, pp. 89–95, 2010.

A. C. C. Egbuonu, O. Obidoa, and C. A. Ezeokonkwo, et al. Some biochemical effects of subacute oral administration of L-arginine on monosodium glutamate fed Wistar albino rats 1: body weight changes, serum cholesterol, creatinine and sodium ion concentrations. Toxicol and Environ Chem, vol. 92, Issue 7, pp. 1331–37, 2010.

A. C. C. Egbuonu, L. U. S. Ezeanyika, P. M. Ejikeme, and O. Obidoa, Histomorphologic alterations in the liver of male Wistar rats treated with l-arginine glutamate and monosodium glutamate. Res. J. Environ. Toxicol, vol. 4, Issue 4, pp. 205-13, 2010.

A. C. C. Egbuonu, P. M. Ejikeme, and N.L. Obasi, Monosodium glutamate: Potentials at inducing prostate pathologies in male Wistar rats. Afr J Biotechnol, vol. 9, Issue 36, pp.5950-54, 2010.

K. Chandra, S. S. Ali, and M. Abid et al. Protection against FCA induced oxidative stress induced DNA damage as a model of arthritis and in vitro antiarthritic potential of Costusspeciosus rhizome extract. Intl J Pharmacognosy and Phytochem Res, vol. 7, Issue 2, pp. 383–89, 2015.

P.D. S. Ventura, C. P. F. Carvalho, and N. M. T. Barros, et al. Malaria infection promotes a selective expression of kinin receptors in murine liver. Malar J, vol.18, Issue 213, pp. 1-10, 2019.

O. Hwang, Role of oxidative stress in Parkinson’s disease.ExpNeurobiol, vol. 22, Issue 1, pp. 11–17, 2013.

C. Roma-Mateo, C. Aguado, G. J. L. Garcia et al., Increased oxidative stress and impaired antioxidant response in lafora disease. Molecular Neurobiol, vol. 51, Issue 3, pp. 932–46, 2015.

P. Byakika-kibwika, M. Lamorde, H. Mayanja-Kizza, S. Khoo, C. Merry, and J. P. van-Geertruyden, Artemether-Lumefantrine Combination Therapy for Treatment of Uncomplicated Malaria: The Potential for Complex Interactions with Antiretroviral Drugs in HIV-Infected Individuals. Malaria Res and Treatment, vol. 2011, pp. 1-5, 2011.

. C. A. Otuechere, G. Edewor, and O. E. Kale, et al., Sub-acute therapeutic dosing of artemether-lumefantrine and artesunate-amodiaquine combination preserves plasma cholesterol, renal antioxidant status, and organ weights in rats. Malaria Res and Treatment, vol. 2012, Issue 2012, pp. 1-5, 2012.

R.J. Little, A. A. Pestano, and Z. Parra, Modeling of peroxide activation in artemisinin derivatives by serial docking. J of Molecular Modeling, vol. 15, pp.847-58, 2009.

World Health Organization (WHO), World malaria report 2014, pp. 1-142, 2014.

O. A. Daikwo, M. U. Kawu, and R. A. Magaji, et al., Effect of prolonged administration of artemether-lumefantrine on testicular biomarkers of oxidative stress: Ameliorative effect of vitamin E. Basic Sciences of Medicine, vol. 7, Issue 1, pp. 1–6, 2018.

S. Abdel-misih, and M. Bloomston, Liver anatomy.Surgical Clinics of North America, vol. 90, Issue 4, pp. 643-53, 2010.

A. C. C. Egbuonu, A. E. Ogbu, and L. U. S. Ezeanyika, Dose-related influence of esculetin (6,7-dihydroxy-coumarin) on some liver and prostate function markers of male Wistar rats. J BiolSci, vol. 12, Issue 4, pp. 253-57, 2012.

M. Zebrowska-Gamdzyk, M. Maciejczyk, and A. Zalewska, et al. Whey protein concentrate WPC-80 intensifies glycoconjugate catabolism and induces oxidative stress in the liver of rats. Nutrients, vol.10, Issue 1178. pp. 1-16. 2018.

A. C. C. Egbuonu, and G. E. Ejike, Effect of pulverized Mangiferaindica(mango) seed kernel on monosodium glutamate-intoxicated rats’ serum antioxidant capacity, brain function and histology. EC Pharmacol. Toxicol, vol. 4, Issue 6, pp. 228-43, 2017.

C. Lopez-Alarcon, and A. Denicola, Evaluating the antioxidant capacity of natural products: A review on chemical and cellular-based assays. Analytical ChimicaActa, vol.763, pp. 1-10, 2013.

O. M. Akanbi, A. B. Odaibo, and K. A. Afolabi, et al., Effect of self-medication with antimalarial drugs on malaria infection in pregnant women in South-Western Nigeria. Medical Principles and Practice, vol. 14, Issue 1, pp. 6–9, 2005.

E. Obi, and A. C. C. Egbuonu, Changes in the liver histomorphology, catalase and glutathione peroxidase activity in the serum and liver homogenate of normal and monosodium glutamate-intoxicated rats co-treated with artemether-lumefantrine. Int J MolBiol Open Access, vol. 4, Issue 2, pp. 67‒73, 2019.

M. M. Gaschler, and B. R. Stockwell, Lipid peroxidation in cell death.Bioch and Biophys Res Commun, vol. 482, Issue 3, pp. 419-25, 2017.

A. A. Omari, C. Gamble, and P. Garner, Artemether-lumefantrine (four-dose regimen) for treating uncomplicated falciparum malaria. Cochrane Database of Systematic Reviews, vol. 2, pp. CD005965, 2006.

A. C. C. Egbuonu, and S. O. Oriji, Pulverized Mangiferaindica(mango) seed kernel mitigated monosodium glutamate-intoxicated rats’ kidney histology and bio-functions. J Nutri Health and FdSci, vol. 5, Issue 2, pp. 1-7, 2017.

U. O. Mbah, and A. C. C. Egbuonu, Ethanolic extract of SolanummelongenaLinn fruit mitigated monosodium glutamate-induced oxidative stress. Intl J Biochem Res & Rev, vol. 18, Issue 2, pp. 1-8, 2017.

U. O. Mbah, and A. C. C. Egbuonu, Ameliorative potentials of egg plant (SolanummelongenaLinn) fruit ethanolic extract on monosodium glutamate intoxicated rats lipid profile, haematology and heart histology. Intl JBiochem Res & Rev, vol. 18, Issue 3, pp. 1-10, 2017.

A. C. C. Egbuonu, and G. A. Ekwuribe, Pulverized Mangiferaindica(mango) seed-kernel modulated serum lipid profile in monosodium glutamate-challenged rats. J ApplBiotechnol, vol. 5, Issue 2, pp. 20-35, 2017.

. B. Wallin, B. Rosengren, H. G. Shertzer and G. Cameyo, Lipoprotein oxidation and measurement of TBARS formation in a single microtiter plate: Its use for evaluation of antioxidants. Anal Biochem, vol. 208, pp. 10-15, 1993.

A. C. C. Egbuonu, and L. U. S. Ezeanyika L.U.S, Effect of L-arginine on selected markers of metabolic syndrome related to oxidative stress, glucose metabolism and nitric oxide synthesis in female Wistar albino rats. Intl Res J of Biochem and Bioinformatics, vol. 2, issue 8, pp. 186-92, 2012.

B. T. Doumas, W. A. Watson, and H. G. Biggs, Albumin standard and the measurement of serum albumin with bromcresol green. ClinChimActa, vol. 3, Issue 1, pp. 87-96, 1971.

A. C. C. Egbuonu, and L. U. S. Ezeanyika, L-arginine exposure improves renal function markers of metabolic syndrome in female rats. Am J of Bioch and MolBiol, vol. 3, Issue 1, pp. 50-60, 2013.

A. C. C. Egbuonu, Effect of some antihypertensives on the serum bilirubin concentration of male Wistar rats. J of Pharmacy and Pharmacol Res, vol. 1, Issue 1, pp. 009-12, 2010.

A. C. C. Egbuonu, D. C. Nzewi, and O. N. C. Egbuonu, Effect of soaking prior to oven-drying on some nutrient and anti-nutrient properties of bitter yam (Dioscoreadumetorum). J NutrFdSci, vol. 4, Issue 280, pp. 1-4,2014.

A. C. C. Egbuonu, D. C. Nzewi, and O. N. C. Egbuonu, Functional properties of bitter yam (Dioscoreadumetorum) as influenced by soaking prior to oven-drying. Am J FdTechnol, vol. 9, Issue 2, pp. 97-03, 2014.

A. C. C. Egbuonu, Effect of ethanolic extract of pulverized Mangiferaindica (mango) seed kernel on some hematological parameters in normal and monosodium glutamate-intoxicated rats. Intl J of Res in Environ Sci, vol. 4, Issue 1, pp. 47-55, 2018.

A. C. C. Egbuonu, C. I. Opara, D. Akachukwu, and U. B. Onyedikachi, Effect of ethanolic extract of avocado pear (Perseaamericana) seed on normal and monosodium glutamate-compromised rats’ hepatic histo-morphology and serum bio-functional parameters. Res J Environ Sci, vol. 12, Issue 2, pp. 53-62, 2018.

T. R. Araujo, I. N. Freitas, J. F. Vettorazzi, T, M. Batista, and J. C. Santos-Silva, et al. Benefits of L-alanine or L-arginine supplementation against adiposity and glucose intolerance in monosodium glutamate-induced obesity. Eur J Nutr, vol. 56, Issue 6, pp. 2069–80, 2017.

M. Andjelkovic, A. B. Djordjevic, E. Antonijevic, and B. Antonijevic, et al. Toxic effect of acute cadmium and lead exposure in rat blood, liver, and kidney. Int. J. Environ. Res. Public Health, 2019, vol. 16, Issue 274; pp. 1-21.

A. Treyer, and A. Musch, Hepatocyte polarity.Comprehensive Physiol, vol. 3, Issue 1, pp.243-87, 2013.

S. Pizzimenti, E. Ciamporcero, M. Daga, P. Pettazzoni, and et al., Interaction of aldehydes derived from lipid peroxidation and membrane proteins. Frontierof Physiol, vol. 4, pp. 242, 2013.

A. C. C. Egbuonu, A. E. Ogbu, I. I. Ijeh, and L. U. S. Ezeanyika, Sub-chronic esculetin (6,7-dihydroxy-coumarin)-induced alteration in some haematological and serum parameters in normal male Wistar rats. Asian J Bioch, vol. 10, Issue 6, pp. 306-11, 2015.

A. Ayala, M. F. Munoz, and S. Arguelles, Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxid Med CellLongev, vol. 2014, Issue 2014, pp. 3604-38, 2014.

V. O. Ukwenya, A. S. Tijani, G. A. Sodunke, and J. B. Fakunle, Acute administration of co-artesiane® induces oxidative stress in the testes of adult male Wistar rats. Biosci Rescommun, vol. 22, Issue 5, pp. 259-65, 2010.

D. G. Levitt, and M. D. Levitt, Human serum albumin homeostasis: a new look at the roles of synthesis, catabolism, renal and gastrointestinal excretion, and the clinical value of serum albumin measurements. Intl J of General Medicine, vol. 9, pp. 229-55, 2016.

P. B. Soeters, R. Robert, R. R. Wolfe, and A. Shenkin, Hypoalbuminemia: Pathogenesis and Clinical Significance. J Parenteral and Enteral Nutr, vol.43, Issue 2, pp. 181-93, 2019.

A. C. C. Egbuonu, and L. U. S. Ezeanyika, Effect of L-arginine on some biochemical markers of metabolic syndrome associated with brain function in female Wistar rats. J Applied Sci, vol. 13, Issue 4, pp. 595-01, 2013.

J. Chi, Q. Xie, J. Jia, and X. Liu, et al., Prognostic Value of Albumin/Globulin Ratio in Survival and Lymph Node Metastasis in Patients with Cancer: A Systematic Review and Meta-Analysis. J of Cancer, vol. 9, Issue 13, pp. 2341-48, 2018.

Y. Yoshino, A. Taguchi, T. Shimizuguchi, Y. Nakajima, and et al., A low albumin to globulin ratio with a high serum globulin level is a prognostic marker for poor survival in cervical cancer patients treated with radiation based therapy. Intl J Gynecol Cancer, vol. 29, Issue 1, pp. 17-22, 2019.

C. Falade, M. Makanga, Z. Premji, C. E. Ortmann, and et al., Efficacy and safety of artemether-lumefantrine (Coartem) tablets (six-dose regimen) in African infants and children with acute, uncomplicated falciparum malaria. Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 99, Issue 6, pp. 459–67, 2005.

S. Darrell, Albumin and its application in drug delivery. Expert Opinion on Drug Delivery, vol. 12, Issue 5, pp. 793-12, 2015.

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Published

2019-12-07

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Section

Graduate Research Articles

How to Cite

[1]
A. C. C. Egbuonu, “Monosodium Glutamate Plus Artemether-lumefantrine Overdose Altered Malondialdehyde, Total Protein and Albumin Concentration in Rats”, Adv. J. Grad. Res., vol. 7, no. 1, pp. 70–79, Dec. 2019.