Monosodium Glutamate Plus Artemether-lumefantrine Overdose Altered Malondialdehyde, Total Protein and Albumin Concentration in Rats

Authors

  • Anthony Cemaluk Chinedum Egbuonu Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria https://orcid.org/0000-0001-5974-415X
  • Emmanuel Obi Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Chinedu P Nwuke Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Cynthia Uchechi Simon Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Justina Utodinachi Oleghibe Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Nnaemeka Raymond Ezenwafor Department of Biochemistry, Michael Okpara University of Agriculture Umudike, Nigeria
  • Ebere Mercy Chukwu Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture Umudike

DOI:

https://doi.org/10.21467/ajgr.7.1.70-79

Abstract

This study aimed at assessing alterations in malondialdehyde, MDA, total protein and albumin concentration in the serum and liver homogenate of monosodium glutamate (MSG)-challenged rats co-treated with artemether-lumefantrine, AL. Methods involving colourimetric estimation were employed in thirty rats randomly grouped into six (n = 5) and for seven consecutive days, fed feed and water (Group A), AL therapeutic dose (Group B), AL overdose (therapeutic dose × 5) (Group C), MSG (8000 mg/kg body weight) (Group D), AL therapeutic dose plus MSG (Group E) or AL overdose plus MSG (Group F). Total protein concentration (2.64±0.09 g/dL, 2.81±0.14g/dL, respectively) in the liver homogenate of rats exposed to MSG (group D) or MSG plus AL overdose (group F) and malondialdehyde concentration in the liver homogenate of MSG plus AL overdose-fed rats (0.45±0.04 mg/ml) lowered (P<0.05) as against other groups. However, serum albumin concentration in MSG (2.59±0.13 g/dl) or AL overdose plus MSG (3.24±0.12 g/dl) fed rats was higher (P<0.05) compared to the control (2.02±0.04 g/dl). The Total protein: Albumin ratio lowered while the Albumin: total protein ratio increased in rats in MSG, AL overdose plus MSG or AL overdose groups compared with the control. Thus, the apparent MSG plus AL overdose-induced adverse influence on the studied parameters and samples of non-malarial infested rats could be via compromised liver-mediated protein metabolism capacity and bio-functions following possibly enhanced protein-malondialdehyde adduct formation in the rats.

Keywords:

Artemesinin-based, therapeutic dose, liver homogenate, malondialdehyde, albumin, total protein

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References

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2019-12-07

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Graduate Research Articles

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[1]
A. C. C. Egbuonu, “Monosodium Glutamate Plus Artemether-lumefantrine Overdose Altered Malondialdehyde, Total Protein and Albumin Concentration in Rats”, Adv. J. Grad. Res., vol. 7, no. 1, pp. 70-79, Dec. 2019.