Comparative Study of the in vitro Antioxidant Properties of Methanolic Extracts of Chromolaena odorata and Ageratum conyzoides used in Wound Healing


  • Babatunde Oso Department of Biochemistry, Kings University, Odeomu
  • Nosarieme Abey Department of Biochemistry, Kings University, Odeomu
  • Oyedotun Oyeleke Department of Biochemistry, Kings University, Odeomu
  • Boyede Olowookere Department of Biochemistry, Kings University, Odeomu



Wound, Antioxidants, Plant extracts, Phytochemicals, Chromolaena odorata, Ageratum conyzoides


This study sought to evaluate the potential antioxidative potencies of Chromolaena odorata and Ageratum conyzoides,  commonly used herbs in Nigeria for wound first aid and healing. The antioxidant potentials were evaluated by measuring their abilities to scavenge radicals, reduce oxidised iron and quench the formation of lipid peroxides. The findings showed that the extract of Ageratum conyzoides exhibited significantly higher (p<0.05) antioxidant potential than the extract of Chromolaena odorata. The study, therefore, suggests that Ageratum conyzoides has higher potential for therapeutic value than Chromolaena odorata in terms of antioxidant potential.


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<li>S. Reddy, R. K. K. Reddy, V. G. Naidu, K. Madhusudhana, S. B. Agwane, S. Ramakrishna and P. V. Diwan, “Evaluation of antimicrobial, antioxidant and wound healing potentials of <em>Holoptelea integrifolia,</em>” <em>J. Ethnopharm.,</em> vol. 115, no. 2, pp. 249–256, 2007.</li>
<li>Y. Mensah, J. Sampson, P. J. Houghton, P. J. Hylands, J. Westbrook, C. M. Dunn, M. A. Hughes and G. W. Cherry, “Effects of <em>Buddleja globosa </em>leaf and its constituents relevant to wound healing,” <em>J. Ethnopharmy</em>., vol. 77, pp. 219–226, 2001.</li>
<li>Süntar, E. K. Akkol, L. Nahar and S. D. Sarker, “Wound healing and antioxidant properties: do they coexist in plants?”&nbsp; <em>Free Rad Antioxid</em>, vol. 2, no. 2, pp. 1-8, 2012.</li>
<li>N. Vaisakh and A. Pandey, “The invasive weed with healing properties: A review on <em>Chromolaena odorata,</em>” <em>Int J Pharm Sci Res.,</em> vol.3, pp. 80–83, 2012.</li>
<li>Gopinath, M. R. Ahmed, K. Gomathi, K. Chitra, P. K. Sehgal and R. Jayakumar, “Dermal wound healing processes with curcumin incorporated collagen films,” <em>Biomaterials</em>, vol. 25, pp. 1911–1917. 2004.</li>
<li>Chow and A. Barbul, “Immunonutrition: Role in Wound Healing and Tissue Regeneration,” Adv Wound Care, vol. 3, no. 1, pp. 46–53, 2014.</li>
<li>Shirwaikar, K. Rajendran and I. S. Punithaa, “<em>In vitro</em> antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine,” <em>Biological and Pharmaceutical Bulletin</em>, vol. 29, pp. 1906–1910, 2006.</li>
<li>J. Ruch, S. J. Cheng and J. E Klaunig, “Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea,” <em>Carcinogenesis,</em> vol. 10, pp. 1003-1008, 1989.</li>
<li>Ruberto, M. T. Baratta, S. G. Deans and H. J. Dorman, “Antioxidant and antimicrobial activity of Feeniculum vulgare and Crithmum maritmum essential oils,”<em> Planta Med</em>., vol. 66, no. 8, pp. 687-693. 2000.</li>
<li>Oyaizu, “Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine,” <em>Jap J Nutr</em>., vol. 44, pp. 307-315, 1986.</li>
<li>Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, “Antioxidant activity applying an improved ABTS&nbsp; radicalcationdecolorization assay,” <em>Free Rad Biol. Med</em>., vol. 26, pp. 1231-1237, 1999.</li>
<li>B. Harborne, “Phytochemical Methods: A guide to Modern Techniques of Plant Analysis,” Chapman and Hall Ltd., London. 1973.</li>
<li>A. T Phan,J. Paterson, M. Bucknall and J. Arcot, “Interactions between phytochemicals from fruits and vegetables: Effects on bioactivities and bioavailability,” <em>Critical Reviews in Food Science and Nutrition</em>, vol. 58, pp. 1310-1329. 2016.</li>
<li>Rashmi and H. G. Rajkumar, “Preliminary Phytochemical Analysis and <em>in vitro</em> Evaluation of Antifungal Activity of Five Invasive Plant Species against Macrophomina Phaseolina (Tassi) Goid,” <em>Int J. Plant Res.,</em> vol. 1, no. 1, pp. 11-15, 2011.</li>
<li>H. Wagner and I. Elmadfa, “Biological relevance of terpenoids: Overview focusing on mono-di and tetraterpenes,” <em>Ann Nutr Metab</em>., vol. 47, pp. 95-106, 2003.</li>
<li>A. Akinpelu, O. A. Igbeneghu, A. I. Awotunde, E. O. Iwalewa and O. O. Oyadapo, “Antioxidant and antibacterial activities of saponin fractions of <em>Erythropheleum suaveolens</em> (Guill. and Perri.) stem bark extract.” <em>Scientific Research and Essays, </em>vol. 9, no. 18, pp. 826-833, 2014.</li>
<li>Prakash, S. Suri, M. Upadhyay and B. N. Singh, “Total phenol, antioxidant and free radical scavenging activities of some medicinal plants,” <em>Int. J. Food Sci. Nutr.,</em> vol. 58, pp. 18–28, 2007.</li>
<li>Piluzza and S. Bullitta, “Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area,” <em>Pharmaceutical Biology,</em> vol. 49, no. 3, pp. 240-247, 2011.</li>




How to Cite

B. Oso, N. Abey, O. Oyeleke, and B. Olowookere, “Comparative Study of the in vitro Antioxidant Properties of Methanolic Extracts of Chromolaena odorata and Ageratum conyzoides used in Wound Healing”, Int. Ann. Sci., vol. 6, no. 1, pp. 8-12, Oct. 2018.



Research Article