Pro-embrionary Somatic Structure of Three Cacao Genotypes (Theobroma Cacao l.) Using Staminodes

  • Mar Asunción Garate-Navarro Instituto de Cultivos Tropicales - ICT
  • Enrique Arévalo-Gardini Instituto de Cultivos Tropicales https://orcid.org/0000-0002-1725-6788
  • Larissa Correa do Bomfim-Costa Universidad Estadual de Santa Cruz – UESC, Brasil
  • Delmira da Costa-Silva Universidad Estadual de Santa Cruz – UESC, Brasil

Abstract

Staminodes of three cocoa genotypes (BT1, ICS-39 and CCN-51), were used to perform the characterization of somatic embryos induced and maintained by DKW culture medium enriched with 2% of sucrose, 10% of coconut water and 0.01% of cysteine, 2,5 mg/l 2,4 D, 5 µg/lTDZ respectively. The ANOVA and the comparison mean test, shown significant differences between globular-, heart-, torpedo- and cotyledonal-stages for each genotype. Additionally, the embryogenic stages and regenerants plants proportions were highest for Blanco Tarapotino 1 (BT1) genotype. A morphological comparison of somatic and zygotic embryos shows similarities in form and symmetry.

Keywords: Tissue culture, tropical crops, in vitro propagation, micro propagation

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References


  1. Cuatrecasas, Cacao and its allies: a taxonomic revision of the genus Theobroma. Contribution from the United States National Herbarium, Washington, v. 35, p. 379-614. 1964.

  2. A. Cooper, J.L. Donovan, A.L.Waterhouse and G. Williamson, Cocoa and health: a decade of research. British. Journal Nutrition, v. 99, p. 1-11. 2007.

  3. Instituto Interamericano de Cooperación para la Agricultura – IICA, Protocolo estandarizado de oferta tecnológica para el cultivo del cacao en Perú. Lima, Perú.

  4. Figueira and J. Janick, Somatic embryogenesis in cacao (Theobroma cacao L.). In: S. Jain, P. Gupta and R. Newton, Somatic embryogenesis in woody plants. Netherlands: Kluwer Academic Publisher, v.2. p.291-310. 1995.

  5. Li, A. Traore, S. Maximova, M.J. Guiltinan, Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron. In Vitro Cellular and Developmental Biology - Plant, v.34, p.293-299, 1998..

  6. Kononowicz, A.K. Kononowicz, J. Janick, Asexual embryogenesis via callus of Theobroma cacao L. Zeitschrift für Pflanzenphysiologie, Stuttgart, v. 113, n. 4, p. 347-358, 1984.

  7. M. Elhag, A. Whipkey and J. Janick, Factors affecting asexual embryogenesis via callus in Theobroma cacao L. Agril. Biol Sci, v. 6, p. 31-43. 1988.

  8. N. Maximova, L. Alemanno, A. Young, N. Ferrière, A. Traoré, M.J. Guiltinan, Efficiency, genotypic variability, and cellular origin of primary and secondary somatic embryogenesis of Theobroma cacao L. In Vitro Cellular & Developmental Biology, v. 38, p. 252-259. 2002.

  9. L. Tan, D.B. Furtek,. Development of an in vitro regeneration system for Theobroma cacao from mature tissues. Plant Science, v. 164, p. 407-412, 2003.

  10. B. Esan, Tissue culture studies on cacao (Theobroma cacao L.): a supplementation of current research. In: INTERNATIONAL COCOA RESEARCH CONFERENCE, v. 5, p.116-125. 1997.

  11. C. Pence, P.M. Hasegawa, J. Janick,  Asexual Embryogenesis in Theobroma cacao L. Journal of American Society for Horticultural Science, v. 104, p. 145-148. 1979.

  12. Wang and C. Janick, Inducing Precocious Germintion in Asexual Embryos of Cacao. Horticultural Sciences, v. 19, p. 839-841. 1984.

  13. J. Novak, G. Owusu, Somatic Embryogenesis and In Vitro Plant Development of Cocoa (Theobroma cacao L.). Nuclear techniques and in vitro culture for plant improvement. IAEA. Vienna, v. 17, p. 443- 449. 1986.

  14. Adu-Ampomah, F.J.  Novak, R. Afza, M. Van Duren and M.  Perea-Dallos. Initiation and growth of somatic embryos of cocoa (Theobroma cacao L.). Cafe, Cacao Thé, v. 32, p.187–197, 1988.

  15.  Omokolo Ndoumou, G. Tsala Ndzomo and N. Niemenak, Phenol Content, Acidic Peroxidase and IAA- oxidase During Somatic Embryogenesis in Theobroma cacao L. Biologia Plantarum, v. 39, p.337-347. 1997.

  16. Driver J.A. & Kuniyuki A.H. (1984) In vitro propagation of paradox walnut rootstock. HortScience 19: 507-509.

  17. N. Maximova, A. Young, S. Pishak and M.J. Guiltinan, Field performance of Theobroma cacao L. plants propagated via somatic embryogenesis. In Vitro Cellular & Developmental Biology, v. 44, p. 487-493. 2008.

  18. J. Guiltinan, S.N. Maximova, College of Agricultural Sciences. Integrated System for Vegetative Propagation of Cacao. Protocol Book. Pennsylvania State University. Version 2.1 4-24pp. 2010

  19. L. Florez, Enhanced somatic embryogenesis in Theobroma cacao using the homologous BABY BOOM transcription factor. BMC Plant Biology, v. 15, p. 121. 2015.

Published
2017-07-07
How to Cite
[1]
M. A. Garate-Navarro, E. Arévalo-Gardini, L. Correa do Bomfim-Costa, and D. da Costa-Silva, “Pro-embrionary Somatic Structure of Three Cacao Genotypes (Theobroma Cacao l.) Using Staminodes”, Int. Ann. Sci., vol. 2, no. 1, pp. 28-32, Jul. 2017.
Section
Short Communication