Induction of Somatic Embryogenesis from Cocoa Farmer Field Collection of ICT - Peru

  • Mar Asunción Garate-Navarro Instituto de Cultivos Tropicales
  • Enrique Arévalo-Gardini Instituto de Cultivos Tropicales - ICT https://orcid.org/0000-0002-1725-6788

Abstract

To induce the formation of embryogenic structures from staminodes, in 10 cocoa genotypes (Theobroma cacao L.) from the ICT Collection, the influence of the combination of two growth hormones (2,4 Dichlorophenoxyacetic 2,4 -D and Thidiazuron TDZ) was studied. Floral explants (staminodes), were induced to form calli in a culture medium supplemented with modified DKW mineral salts. At 12 days of implantation in the culture medium, the explants responded to the callus induction with a bulge at the base, covering the explants totally with callus at 40 days of culture. Embryogenic expression was shown at 52 days of culture. The genotypes ICT-2161 and ICT-2142 shows the highest percentage of somatic embryogenesis. Four types of callus, fine crystalline, thick crystalline, semi-compact and compact were observed. In addition, a clear organogenic response was observed when roots were formed from the callus generated.

Keywords: Tissue culture, tropical crops, in vitro propagation, micro propagation

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Author Biography

Enrique Arévalo-Gardini, Instituto de Cultivos Tropicales - ICT

Coordinador General, Instituto de Cultivos Tropicales (ICT), Av Ahuashiyacu S/N, Cuadra 16, Sector Laguna Venecia, Banda del Shilcayo
Referencia( Esquina Esquina Mz. L, Urb. Santa Barbara y Ahuashiaco), Tarapoto, San Martín, Perú, Casilla Postal SERPOST Nº 201

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Published
2017-06-30
How to Cite
[1]
M. A. Garate-Navarro and E. Arévalo-Gardini, “Induction of Somatic Embryogenesis from Cocoa Farmer Field Collection of ICT - Peru”, Int. Ann. Sci., vol. 2, no. 1, pp. 6-11, Jun. 2017.
Section
Research Article