Toxicological Safety Assessment of Molluscicides Against Non-target Aquatic Biota; Colisa fasciatus

  • Neelam Soni Din Dayal Upadhyay Gorakhpur University, Gorakhpur
  • Vinay Kumar Singh Malacology laboratory, Department of Zoology, DDU Gorakhpur University Gorakhpur


Plants Terminalia arjuna and Tamarindus indica are known to have a significant molluscicidal potential to control the population of vector snails. Since the molluscicides are considered an emerging pollutant and are frequently detected in surface water bodies and found to be a great threat for aquatic biota. Hence, the main objective of this research is to critically evaluate the ectotoxicological and chronic effect of plant-derived molluscicides on other aquatic biotas. With these references this study deals with the safety measurement of molluscicides arjnolic acid, saponin and procynadine from T. arjuna and T. Indica against the fish Colisa fasciatus which share the same habitat with snails. The result of toxicity experiment reveals that fishes showed no mortality against 24h LC90 (against L. acuminata and I. exustus) up to 96h exposure duration. The enzyme bioassays of these molluscicides on the nervous tissue of fish showed no significant effect on key enzymes Acetylcholinesterase, acid and alkaline phosphates activity in comparison to control group of fishes. These results indicated that the application of arjunolic acid, saponin, and procynadine derive from plant T. arjuna and T. indica at its maximum concentration (24h LC90 of L. acuminata and I. exustus) and exposure duration (96h) did not cause any mortality or treatment-related enzymes inactivity in fishes. The study conclusively proved the ecotoxicological and chronic safety of plant-derived molluscicides arjunolic acid, saponin, and procynadine on non-target animals in the aquatic environment.

Keywords: Molluscicides; Fish; Ectotoxicology; snails; Plants; Environmental safety; Snails


Download data is not yet available.


[1]  M. Meshesha and W.  Tesfaye, “Prevalence of fasciolosis in the cattle slathered at Honsana Muncipal Abattoir, Southern Ethiopia” International Journal of Advance Research in Biological Science, vol. 4, no. 2, pp 40-46, 2017.

[2]  N. Soni and V.K. Singh, “Molluscicidal activity of Tamarindus indica and Terminalia arjuna against Indoplanorbis exustus: A causative agent of Trematodiasis. Scientia Agriculture, vol.12, pp 163-170, 2015

[3]    N. Soni and V.K. Singh, “Screening of molluscicidal potential of indigenous medicinal plants Terminalia arjuna and Tamarindus indica against fasciolosis vector: Lymnaea acuminata”. Asian Journal of Science and Technology. Vol. 8 no. 8, pp5256-5261. 2017.

[4]  D. Chaturvedi and V.K. Singh,   “Toxicity of Chlorophylline against Lymnaea acuminata at different wavelength of visible light”. Tropical Life Sciences Research,  vol. 27 no. 2, pp 25-36, 2016.

[5]  K. Singh, D.K.Singh, and  V. K. Singh, “Chlorophylline treatment against Lymnaea acuminata: a new tool in fasciolosis control”. Pharmacognosy Journal, vol. 9 no. 5, pp 594-598.2017.

[6]   S.L. Kela, R.A. Oqunsusi, V.C. Ogubogu and N. Nwude, “Screening of some Nigerians plants for molluscicidal activity” Revue d’ elevagave et de  medicine veterinaire des  pays tropicaux,  vol. 42no. 2, pp 195-202.1989.

[7]  B.B Mosta-fa, F.A. El-deeb, N.M Ismail  and K.M. el-Said, “Impact of some plant and synthetic molluscicides on fresh water snails and fish”. Journal of Egyptian Society of Parasitology, vol. 35 no. 3, pp 898-1007.2005

[8]  A. Amalraj and S. Gopi, “Medicinal properties of Terminalia arjuna (Roxb) Wight & Arn”. Journal of Traditional and Complimentary Medicine,  vol. 7, pp 65-78.2017

[9] J.A Riberio, A.C. Serquiz, P.F. Silva, P.B. Barbosa, T.B. Sampaio, R.F. Araujo junior A.S. Oliveira, R.J. Machado, B.L. Maciel, A.F.mUchoa, E.A. Santos, and A.H. Morais, “Trypsin inhibitor from Tamarindus indica seeds Linn. reduced weight gain cholecystokinin levels". Clinics (Sao Paulo), vol. 70 no. 2,  pp136.2015

[10]  T. Javed, S. Riaz, M. Uzair, G. Mustafa, A. Mohyuddin and B.C. Ahamad, “Biological activity of Terminalia arjuna on human pathogenic microorganisms.” Pakistan Journal of Pharmacology Research, vol, 2 no. 1, pp  23.2016

[11]   S. Zohrameena, M. Mujahid, P. Bagga, M. Khalid, H. Noorul, A. Nesar, and P. Saba, “Medicinal uses and pharmacological activity of Tamarindus indica”. World Journal of Pharmacological Science, vol. 5 no. 2), pp. 121-137.2017

[12] N. Soni, D.K. Singh and V.K. Singh, “Inhibition kinetics of acetylcholinesterase and phosphatises by the active constituents of Terminalia arjuna and Tamarindus indica in the cerebral ganglion of Lymnaea acuminata”. Pharmacognosy  Journal,   vol 9 . no.2, pp148-156.

[13]   N. Soni, D.K. Singh and V.K. Singh, “HPLC characterization of molluscicidal component of Tamarindus indica and its mode of action on nervous tissue of Lymnaea acuminata”  journal of Ayurveda and Integrative Medicine. {online first),1-11 Elsevier. IF-0.98.2018

[14] G.L. Ellman, K.D. Courtney, V. Andres, and R.M.A. Featherstone, “New rapid colorimetric determination of acetylcholinesterase activity” Biochemistry Pharmacology, vol. 7, pp88-95.1961

[15] U.H. Bergmeyer, “Methods of enzymatic analysis” Academic Press:  New York, pp1129.1967.

[16] O.H. Lowry, N.J. Rosenbrough, A.L. Farr and R.J. Randall, “Protein measurement with the folin phenol reagents” Journal of Biological Chemistry, vol. 193, pp. 265-275.1951

[17] R.R. Sokal, and F.J. Rohlf, “Introduction to Biostatistics” San Francisco: W.H. Freeman.pp 225-229.1955

[18] A. Prakash, J. Rao, and V. Nandagopal,  “Managing resistance to insecticides is a key for the future of crop protection”  Journal of  Biopesticides, vol 1, pp.154–169.2008

[19]   U. Istvan, “Semi-natural products and related substances and alleged botanical pesticides” Pest Management Science , vol. 56 no. 8, pp, 703-705.2000.

[20] J. Shahi and A. Singh, “Comparative study on piscicidal activity of synthetic pesticides and plant origin piscicides, to fish Channa punctatus”. World Journal of Zoology, vol. 5 no.1, pp. 20-24.2010.

[21]  D. Chaturvedi and V.K. Singh, “Assessment the effect of photodynamic Chlorophylline on biochemical changes in the cerebral ganglion of Lymnaea acuminata” International  Journal of Pharma Science and  Research.  Vol. 8, no.5, pp 68-75.2017

 [22] G. Prabhakaran, S.J. Bhore, and M. Ravichandran, “Development and evaluation of poly herbal molluscicides extract for control of apple snail (Pomacea maculata)”. Agriculture, vol. 7, pp. 22, 2017.

How to Cite
N. Soni and V. Singh, “Toxicological Safety Assessment of Molluscicides Against Non-target Aquatic Biota; Colisa fasciatus”, Int. Ann. Sci., vol. 7, no. 1, pp. 21-27, Apr. 2019.
Research Article