Antioxidant and Antimicrobial Properties of Ocimum sanctum and Cymbopogon nardus

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DOI:

https://doi.org/10.21467/ajgr.9.1.14-20

Abstract

Plant extracts have gained popularity recently, for their importance as potential antioxidative and antimicrobial agents. These properties have been attributed to their phytochemical content. The extraction solvent and the plant part are among the factors that influence the yield of these phytochemicals This study was therefore undertaken to investigate the antimicrobial and antioxidant activities of extracts of the leaf and stem of Ocimum sanctum (holy basil) and Cymbopogon nardus (citronella grass); two commonly occurring plants in South East Asia. The extracts were obtained by solvent extraction using water, methanol and ethanol. The percentage yield, antimicrobial activity, antioxidant activity and high-performance liquid chromatography (HPLC) analysis was investigated. The organic extracts yielded a higher percentage recovery of phytochemicals compared to the water extracts.  HPLC analysis revealed the presence of chlorogenic acid in all extracts; rutin only in the leaf extracts and the citronella grass leaf extract contained both rutin and gallic acid. Antimicrobial assays were performed using the agar well diffusion method with tetracycline as positive control. Basil extracts exerted a greater inhibitory growth on both S. aureus and E. coli. S. aureus was found to be more susceptible to the presence of plant extracts. Water extracts did not display any zones of inhibition. The DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was used to study the antioxidant effect with Vitamin C (0.1mg/ml) as positive control. The results indicated that the Basil leaf extracts possessed greater antioxidant potential compared to the stem. The study concludes that organic extracts of O. sanctum and C. nardus possess pharmaceutical properties.

Keywords:

Antimicrobial, Antioxidant, Cymbopogon nardus, Ocimum sanctum

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References

H.A Yamani., E.C Pang., N. Mantri., & M.A Deighton. “Antimicrobial Activity of Tulsi (Ocimum sanctum) Essential Oil and Their Major Constituents against Three Species of Bacteria”, Frontiers in Microbiology,7, 681. http://doi.org/10.3389/fmicb.2016.00681, May 2016.

P. Subramanian., C. W Imanina., C.W. Takwa., N. E. A. Zubair. “Chemical composition and antibacterial activity of essential oil of Cymbopogon citratus and Cymbopogon nardus against Enterococcus faecalis”, International Journal of Biosciences Vol. 6, No. 9, p. 9-17, May 2015.

S. Bhattacharya. “Free Radicals in Human Health and Disease; Department of Biotechnology”, Springer, India, India, http://dx.doi.org/10.1007/978-81-322-2035-0_2. Jun 2015.

P. Yudharaj, M. Shankar*, R. Sowjanya, B. Sireesha, E. Ashok Naik, R. Jasmine Priyadarshini. “Importance and uses of Medicinal Plants – an Overview”, Int. J. Preclinical & Pharm. Res. 7. pp 67-73 Dec 2016

C. Lee Ventola. “The Antibiotic Resistance Crisis, Part 1: Causes and Threats”. Article in P&T, 40(4): 277–283. PMC4378521. April 2015

I. Cock.., M.J. Cheesman., A. Ilanko., B. Blonk. “Developing new antimicrobial therapies: Are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution”, Pharmacogn.;11:57–72. July 2017

P. Ruban., K. Gajalakshmi. “In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens”, Asian Pac J Trop Biomed;2:399–403, May 2012.

P Eswar., C.G. Devaraj., P. Agarwal. “Anti-microbial Activity of Tulsi {Ocimum Sanctum (Linn.)} Extract on a Periodontal Pathogen in Human Dental Plaque: An Invitro Study, J Clin Diagn” Res;10(3): p53–56, Mar 2016.

M.. Işık., S. Beydemir., A. Yılmaz., M.E. Naldan., H.E. Aslan., İ. Gülçin. “Oxidative stress and mRNA expression of acetylcholinesterase in the leukocytes of ischemic patients”, Biomed. Pharmacother; 87:561–567. doi: 10.1016/j.biopha.2017.01.003, Mar 2015.

V.R. de Souza. , P.A.P Pereira., T.L.T. da Silva., L.C.O. Lima. , R.P.F Queiroz. “Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits”. Food Chemistry, pages 362-368, Aug 2014.

M. Liaudanskas, K. Zymonė. J. Viškelis, A. Klevinskas, and V. Janulis. “Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts”, Journal of Chemistry, ArticleID7856521, 9pages, May 2017. https://doi.org/10.1155/2017/7856521

C.Y. Huang, C.H. Kuo, C.H.Wu, A.W. Kuan, H.R. Guo, Y.H. Lin, and P.K. Wang. “Free Radical-Scavenging, Anti-Inflammatory, and Antibacterial Activities of Water and Ethanol Extracts Prepared from Compressional-Puffing Pretreated Mango (Mangifera indica L.) Peels”, Journal of Food Quality Article ID 1025387, 13 pages, Feb 2018. https://doi.org/10.1155/2018/1025387

D.H Truong., D.H Nguyen., N.T.A Ta., A.V Bui., T.H Do., & H.C Nguyen. “Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia”. Journal of Food Quality. Article ID 8178294 | 9 pages. May 2019

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Published

2020-09-21

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Section

Graduate Research Articles

How to Cite

[1]
R. A. Ogbonna, R. Ramanathan, and N. S. Ping, “Antioxidant and Antimicrobial Properties of Ocimum sanctum and Cymbopogon nardus”, Adv. J. Grad. Res., vol. 9, no. 1, pp. 14-20, Sep. 2020.