Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors


  • Arif Rahmadi Study Program of Marine Science, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java 45363 Indonesia
  • Yeni Mulyani Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java 45363 Indonesia
  • Muhammad Wahyudin Lewaru Laboratory of Microbiology and Molecular Biotechnology (MICROMOL), Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java 45363 Indonesia




Microalgae, Chaetoceros, Photobioreactor, lipid


Chaetoceros muelleri is a microalgae class of Bacillariophyta (diatom) which is generally only used as feeds for fishes and shellfish larvae. Nevertheless, the biochemical content of this species is quite high and has the potential to be developed. This research aims to explain the effect of different salinity on the growth and lipid content of Chaetoceros muelleri cultured in a continuous photobioreactor. This research was carried out in August 2018 - February 2019. The research was conducted at the Laboratory of Marine Microbiology and the Laboratory of Bioprocess and Bioprospection of Natural Materials, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The samples of Chaetoceros muelleri isolates were obtained from the Jepara Brackish Water Aquaculture Center. The methods used for the study was a ‘Completely Randomized Design’ (CRD) with four treatments. The salinity used is 15, 25, 35 and 45 ppt. The main parameters observed were growth and lipid content, while the supporting parameters were temperature, and pH. The results of this study showed that the highest lipid content was a salinity treatment of 35 ppt with a value of 25.37% of total dry weight obtained at the end of the culture. Based on growth, the highest density occurred in 25 ppt salinity with a maximum density of 3.80 ± 0.49 x 106 cells. ml-1 and maximum growth rate of 0.36 ± 0.008 div. day-1


Download data is not yet available.


<p>[1]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Chaisutyakorn, P., Praiboon, J. &amp; Kaewsuralikhit, C., “The effect of temperature on growth and lipid and fatty acid composition on marine microalgae used for biodiesel production”. <em>J Appl Phycol</em>, pp.1–9. 2017.</p>
<p>[2]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Barros, M.U.G. et al., “Lipid content of marine microalgae Chaetoceros muelleri Lemmermann (Bacillariophyceae) grown at different salinities”. <em>Biotemas</em>, vol. 27, no. 2, pp.1–8. 2014.</p>
<p>[3]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Smodlaka Tanković, Mirta et al. “Insights into the life strategy of the common marine diatom Chaetoceros peruvianus Brightwell.” <em>PloS one.</em> vol. 13, no.9e0203634. 12 Sep. 2018.</p>
<p>[4]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Jamali, H., Ahmadifard, N. &amp; Abdollahi, D. “Evaluation of growth, survival and body composition of larval white shrimp (Litopenaeus vannamei) fed the combination of three types of algae”. Int Aquat Res. vol.115, no.7. 2015.</p>
<p>[5]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Benavente-valdés, J.R. et al. “Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species”. <em>Biotechnology Report</em>, vol.10, pp.117–125. 2016.</p>
<p>[6]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Siqueira, S. F., Francisco, É. C., Queiroz, M. I., Menezes, C. R. de, Zepka, L. Q., &amp; Jacob-Lopes, E. “Third Generation Biodiesel Production From Microalgae Phormidium autumnale”. <em>Brazilian Journal of Chemical Engineering</em>, vol.33, no.3, pp. 427-433. 2016.</p>
<p>[7]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; BenMoussa-Dahmen, I., Chtourou, H., Rezgui, F., Sayadi, S., &amp; Dhouib, A.. “Salinity stress increases lipid, secondary metabolites and enzyme activity in Amphora subtropica and Dunaliella sp. for biodiesel production”. <em>Bioresource Technology</em>, &nbsp;vol. 218, pp.816–825. 2016.</p>
<p>[8]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Ishika, T., Moheimani, N. R., Bahri, P. A., Laird, D. W., Blair, S., &amp; Parlevliet, D. . “Halo-adapted microalgae for fucoxanthin production: Effect of incremental increase in salinity”. <em>Algal Research</em>, vol. 28, pp.66–73. 2017.</p>
<p>[9]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Ji, X., Cheng, J., Gong, D., Zhao, X., Qi, Y., Su, Y., &amp; Ma, W. “The effect of NaCl stress on photosynthetic efficiency and lipid production in freshwater microalga— Scenedesmus obliquus XJ002”. <em>Science of The Total Environment</em>, vol. 633, pp. 593–599. 2018.</p>
<p>[10]&nbsp;&nbsp;&nbsp;&nbsp; Du, Z.-Y., &amp; Benning, C. Triacylglycerol Accumulation in Photosynthetic Cells in Plants and Algae. Lipids in Plant and Algae Development, 179–205. 2016.</p>
<p>[11]&nbsp;&nbsp;&nbsp;&nbsp; Pancha, I., Chokshi, K., Maurya, R., Trivedi, K., Patidar, S. K., Ghosh, A., &amp; Mishra, S. “Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077”. <em>Bioresource Technology</em>, vol.189, pp. 341–348. 2015.</p>
<p>[12]&nbsp;&nbsp;&nbsp;&nbsp; Paes , Caroline R.P.S. et al. “Growth, nutrient uptake and chemical composition of Chlorella sp. and Nannochloropsis oculataunder nitrogen starvation”. <em>J.Aquatic</em>. vol. 42, no. 2, pp.275-292. 2016.</p>
<p>[13]&nbsp;&nbsp;&nbsp;&nbsp; Paliwal, C., Mitra, M., Bhayani, K., Bharadwaj, S. V. V., Ghosh, T., Dubey, S., &amp; Mishra, S. “Abiotic stresses as tools for metabolites in microalgae”. <em>Bioresource Technology</em>, vol. 244, pp.1216–1226. 2017.</p>
<p>[14]&nbsp;&nbsp;&nbsp;&nbsp; Houslow, Emily. et al. 2016. “The Search for a lipid trigger : The Effect of salt stress on the lipid profile of model microalgal species chlamydomonas reinhardtii for biodiesel production”. <em>Current Biotechnology.</em> vol. 5, pp.305-313. 2016.</p>
<p>[15]&nbsp;&nbsp;&nbsp;&nbsp; Jannah, M., Ulkhaq, M. F., Azhar, M. H., Suciyono, &amp; Soemarjati. “Growth Performance of Laboratory-Scale Chaetoceros calcitrans in Different Containers”. <em>IOP Conference Series: Earth and Environmental Science</em>, vol. 236, No. 012031, 2019.</p>




How to Cite

A. Rahmadi, Y. Mulyani, and M. W. Lewaru, “Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors”, Adv. J. Grad. Res., vol. 7, no. 1, pp. 3-10, Aug. 2019.



Graduate Research Articles